|
KMID : 1041219970390020154
|
|
Journal of Sericultural and Entomological Science
1997 Volume.39 No. 2 p.154 ~ p.160
|
|
|
Establishment of Simplistic Moth Inspection System to Prevent Nosema bombycis Infection of the Silkworm, Bombyx mori
|
|
Han, Myung Sae
Mau Tuan Nguyen/Lim, Jong Sung
|
|
|
Abstract
|
|
|
|
Present experiment designed for the review of theoretical basis for the inspertion system of infected insects by Noema bombycis. A microsporidian N. bombycis, known as the high virulence, produced at the average of 7 X 108 spores per female moth of the silkworm, Bombyx mori, enabled transovarial tranmission. Detectability of N. bombycis spores in the mass inspection was varied by dillution level, the higher limit of dillution with healthy moths was 1:140 for 100% detection, 1:160 for 99.5%, 1:200 for 99.0%, For an efficient inspection under the microdcopic observation (600 X ), the lower limit of spore concentration was determined as 1,000,000 spores/ml, 60~80 moths could be applicable for a maximunl sample unit of a lot. Following the present inspection unit conditioned 35 to 40 moths for a lot. N. bombycis spores were easily detectable from the preparation of crude homogenate with 2% KOH, even the step of centrifuge was omitted. The results suggested a new basis of rational mass inspection system of silkworm female moths to save the facilities, labor, and time
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|